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The SARS coronavirus accessory protein ORF3a rescues potassium conductance in yeast.
- Source :
-
MicroPublication biology [MicroPubl Biol] 2024 Mar 07; Vol. 2024. Date of Electronic Publication: 2024 Mar 07 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- ORF3a is an accessory protein expressed by all human pathogen coronaviruses and is the only accessory protein that strongly affects viral fitness. Its deletion reduces severity in both alpha- and beta-coronaviruses, demonstrating a conserved function across the superfamily. Initially regarded as a non-selective cation channel, ORF3a's function is now disputed. Here, we show that ORF3a from SARS, but not SARS-CoV-2, promotes potassium conductance in a yeast model system commonly used to study potassium channels. ORF3a-mediated potassium conductance is also sensitive to inhibitors, including emodin, carbamazepine, and nifedipine. This model may be used in future studies on ORF3a and related proteins.<br />Competing Interests: The authors declare that there are no conflicts of interest present.<br /> (Copyright: © 2024 by the authors.)
Details
- Language :
- English
- ISSN :
- 2578-9430
- Volume :
- 2024
- Database :
- MEDLINE
- Journal :
- MicroPublication biology
- Publication Type :
- Academic Journal
- Accession number :
- 38525126
- Full Text :
- https://doi.org/10.17912/micropub.biology.001129