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Multiple SigB homologues govern the transcription of the ssgBp promoter in the sporulation-specific ssgB gene in Streptomyces coelicolor A3(2).
- Source :
-
Research in microbiology [Res Microbiol] 2024 Jul-Aug; Vol. 175 (5-6), pp. 104201. Date of Electronic Publication: 2024 Mar 24. - Publication Year :
- 2024
-
Abstract
- Unlike Bacillus subtilis, Streptomyces coelicolor contains nine SigB homologues of the stress-response sigma factor SigB. By using a two-plasmid system, we previously identified promoters recognized by these sigma factors. Almost all promoters were recognized by several SigB homologues. However, no specific sequences of these promoters were found. One of these promoters, ssgBp, was selected to examine this cross-recognition in the native host. It controls the expression of the sporulation-specific gene ssgB. Using a luciferase reporter, the activity of this promoter in S. coelicolor and nine mutant strains lacking individual sigB homologous genes showed that sgBp is dependent on three sigma factors, SigH, SigN, and SigI. To determine which nucleotides in the-10 region are responsible for the selection of a specific SigB homologue, promoters mutated at the last three nucleotide positions were tested in the two-plasmid system. Some mutant promoters were specifically recognized by a distinct set of SigB homologues. Analysis of these mutant promoters in the native host showed the role of these nucleotides. A conserved nucleotide A at position 5 was essential for promoter activity, and two variable nucleotides at positions 4 and 6 were responsible for the partial selectivity of promoter recognition by SigB homologues.<br />Competing Interests: Declaration of competing interest The authors declare no competing interests.<br /> (Copyright © 2024 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Plasmids genetics
Base Sequence
Streptomyces coelicolor genetics
Streptomyces coelicolor metabolism
Promoter Regions, Genetic
Sigma Factor genetics
Sigma Factor metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Spores, Bacterial genetics
Spores, Bacterial metabolism
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1769-7123
- Volume :
- 175
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Research in microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38522628
- Full Text :
- https://doi.org/10.1016/j.resmic.2024.104201