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Biosynthesis of mannose from glucose via constructing phosphorylation-dephosphorylation reactions in Escherichia coli.
- Source :
-
Enzyme and microbial technology [Enzyme Microb Technol] 2024 Jun; Vol. 177, pp. 110427. Date of Electronic Publication: 2024 Mar 18. - Publication Year :
- 2024
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Abstract
- d-mannose has been widely used in food, medicine, cosmetic, and food-additive industries. To date, chemical synthesis or enzymatic conversion approaches based on iso/epimerization reactions for d-mannose production suffered from low conversion rate due to the reaction equilibrium, necessitating intricate separation processes for obtaining pure products on an industrial scale. To circumvent this challenge, this study showcased a new approach for d-mannose synthesis from glucose through constructing a phosphorylation-dephosphorylation pathway in an engineered strain. Specifically, the gene encoding phosphofructokinase (PfkA) in glycolytic pathway was deleted in Escherichia coli to accumulate fructose-6-phosphate (F6P). Additionally, one endogenous phosphatase, YniC, with high specificity to mannose-6-phosphate, was identified. In ΔpfkA strain, a recombinant synthetic pathway based on mannose-6-phosphate isomerase and YniC was developed to direct F6P to mannose. The resulting strain successfully produced 25.2 g/L mannose from glucose with a high conversion rate of 63% after transformation for 48 h. This performance surpassed the 15% conversion rate observed with 2-epimerases. In conclusion, this study presents an efficient method for achieving high-yield mannose synthesis from cost-effective glucose.<br />Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Phosphorylation
Escherichia coli Proteins metabolism
Escherichia coli Proteins genetics
Mannosephosphates metabolism
Metabolic Engineering
Fructosephosphates metabolism
Mannose-6-Phosphate Isomerase metabolism
Mannose-6-Phosphate Isomerase genetics
Phosphoric Monoester Hydrolases metabolism
Phosphoric Monoester Hydrolases genetics
Glycolysis
Mannose metabolism
Escherichia coli genetics
Escherichia coli metabolism
Glucose metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0909
- Volume :
- 177
- Database :
- MEDLINE
- Journal :
- Enzyme and microbial technology
- Publication Type :
- Academic Journal
- Accession number :
- 38518553
- Full Text :
- https://doi.org/10.1016/j.enzmictec.2024.110427