Back to Search
Start Over
Lipid metabolism-related long noncoding RNA RP11-817I4.1 promotes fatty acid synthesis and tumor progression in hepatocellular carcinoma.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2024 Feb 28; Vol. 30 (8), pp. 919-942. - Publication Year :
- 2024
-
Abstract
- Background: Hepatocellular carcinoma (HCC) is one of the most common types of tumors. The influence of lipid metabolism disruption on the development of HCC has been demonstrated in published studies.<br />Aim: To establish an HCC prognostic model for lipid metabolism-related long non-coding RNAs (LMR-lncRNAs) and conduct in-depth research on the specific role of novel LMR-lncRNAs in HCC.<br />Methods: Correlation and differential expression analyses of The Cancer Genome Atlas data were used to identify differentially expressed LMR-lncRNAs. Quantitative real-time polymerase chain reaction analysis was used to evaluate the expression of LMR-lncRNAs. Nile red staining was employed to observe intracellular lipid levels. The interaction between RP11-817I4.1, miR-3120-3p, and ATP citrate lyase (ACLY) was validated through the performance of dual-luciferase reporter gene and RIP assays.<br />Results: Three LMR-lncRNAs (negative regulator of antiviral response, RNA transmembrane and coiled-coil domain family 1 antisense RNA 1, and RP11-817I4.1) were identified as predictive markers for HCC patients and were utilized in the construction of risk models. Additionally, proliferation, migration, and invasion were reduced by RP11-817I4.1 knockdown. An increase in lipid levels in HCC cells was significantly induced by RP11-817I4.1 through the miR-3120-3p/ACLY axis.<br />Conclusion: LMR-lncRNAs have the capacity to predict the clinical characteristics and prognoses of HCC patients, and the discovery of a novel LMR-lncRNAs, RP11-817I4.1, revealed its role in promoting lipid accumulation, thereby accelerating the onset and progression of HCC.<br />Competing Interests: Conflict-of-interest statement: There are no conflicts of interest to disclose in this article.<br /> (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Subjects :
- Humans
Lipid Metabolism genetics
Fatty Acids
Lipids
Gene Expression Regulation, Neoplastic
Cell Proliferation genetics
Cell Line, Tumor
Carcinoma, Hepatocellular genetics
Carcinoma, Hepatocellular pathology
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Liver Neoplasms genetics
Liver Neoplasms pathology
MicroRNAs genetics
MicroRNAs metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 38516243
- Full Text :
- https://doi.org/10.3748/wjg.v30.i8.919