Ultrasensitive detection of zearalenone based on electrochemiluminescent immunoassay with Zr-MOF nanoplates and Au@MoS 2 nanoflowers.

In this work, an effective competitive-type electrochemiluminescence (ECL) immunosensor was constructed for zearalenone determination by using Zr-MOF nanoplates as the ECL luminophore and Au@MoS ₂ nanoflowers as the substrate material. Zr-MOF have an ultra-thin sheet-like structure that accelerates the transfer of electrons, ions and co-reactant intermediates, which exhibited strong and stable anodic luminescence. The three-dimensional Au@MoS ₂ nanoflowers would form a thin film modification layer on the glassy carbon electrode (GCE). And its good electrical conductivity and higher specific surface area utilization further improving the sensitivity of the ECL immunosensor. Under the optimized conditions, the proposed immunosensor exhibited satisfactory stability, sensitivity and accuracy, and its ECL signal was proportional to the logarithm of ZEN concentration (0.0001-100 ng/mL) and the limit of detection (LOD) was 0.034 pg/mL. In addition, the results of recovery experiment acquired for wheat flour and pig urine samples further proved the feasibility of the immunosensor for the detection of real samples, indicating its potential for ultrasensitive detection of ZEN.
Competing Interests: Declaration of competing interest All the authors listed have approved the submission: The first author Lu Peng declares that she has no conflict of interest. The second author Xinyue Qian declares that she has no conflict of interest. The third author Ya Jin declares that she has no conflict of interest. The fourth author Xiangyang Miao declares that he has no conflict of interest. The fifth author Anping Deng declares that he has no conflict of interest. Corresponding Author Jianguo Li declares that he has no conflict of interest. Informed consent was obtained from all individual participants included in the study.
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