Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.

The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
Competing Interests: Declaration of interests This research is partly funded by a grant from ElmediX NV. T.L. and E.v.Z. are employees of ElmediX. J.B. is the CEO and a shareholder of ElmediX NV.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)