Back to Search
Start Over
Hierarchically Biomimetic Scaffolds with Anisotropic Micropores and Nanotopological Patterns to Promote Bone Regeneration via Geometric Modulation.
- Source :
-
Advanced healthcare materials [Adv Healthc Mater] 2024 Jul; Vol. 13 (17), pp. e2304178. Date of Electronic Publication: 2024 Mar 22. - Publication Year :
- 2024
-
Abstract
- Structural engineering is an appealing means to modulate osteogenesis without the intervention of exogenous cells or therapeutic agents. In this work, a novel 3D scaffold with anisotropic micropores and nanotopographical patterns is developed. Scaffolds with oriented pores are fabricated via the selective extraction of water-soluble polyethylene oxide from its poly(ε-caprolactone) co-continuous mixture and uniaxial stretching. The plate apatite-like lamellae are subsequently hatched on the pore walls through surface-induced epitaxial crystallization. Such a unique geometric architecture yields a synergistic effect on the osteogenic capability. The prepared scaffold leads to a 19.2% and 128.0% increase in the alkaline phosphatase activity of rat bone mesenchymal stem cells compared to that of the scaffolds with only oriented pores and only nanotopographical patterns, respectively. It also induces the greatest upregulation of osteogenic-related gene expression in vitro. The cranial defect repair results demonstrate that the prepared scaffold effectively promotes new bone regeneration, as indicated by a 350% increase in collagen I expression in vivo compared to the isotropic porous scaffold without surface nanotopology after implantation for 14 weeks. Overall, this work provides geometric motifs for the transduction of biophysical cues in 3D porous scaffolds, which is a promising option for tissue engineering applications.<br /> (© 2024 Wiley‐VCH GmbH.)
- Subjects :
- Animals
Rats
Porosity
Anisotropy
Tissue Engineering methods
Polyesters chemistry
Rats, Sprague-Dawley
Biomimetic Materials chemistry
Biomimetic Materials pharmacology
Male
Alkaline Phosphatase metabolism
Skull
Tissue Scaffolds chemistry
Bone Regeneration drug effects
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Osteogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2192-2659
- Volume :
- 13
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Advanced healthcare materials
- Publication Type :
- Academic Journal
- Accession number :
- 38490686
- Full Text :
- https://doi.org/10.1002/adhm.202304178