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Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2024 Mar; Vol. 170 (3). - Publication Year :
- 2024
-
Abstract
- Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria to utilise Sia for growth and/or cell surface modification. While N-acetyl-neuraminic acid (Neu5Ac)-specific transporters have been studied extensively, little is known on transporters dedicated to anhydro-Sia forms such as 2,7-anhydro-Neu5Ac (2,7-AN) or 2,3-dehydro-2-deoxy-Neu5Ac (Neu5Ac2en). Here, we used a Sia-transport-null strain of Escherichia coli to investigate the function of members of anhydro-Sia transporter families previously identified by computational studies. First, we showed that the transporter NanG, from the Glycoside-Pentoside-Hexuronide:cation symporter family, is a specific 2,7-AN transporter, and identified by mutagenesis a crucial functional residue within the putative substrate-binding site. We then demonstrated that NanX transporters, of the Major Facilitator Superfamily, also only transport 2,7-AN and not Neu5Ac2en nor Neu5Ac. Finally, we provided evidence that SiaX transporters, of the Sodium-Solute Symporter superfamily, are promiscuous Neu5Ac/Neu5Ac2en transporters able to acquire either substrate equally well. The characterisation of anhydro-Sia transporters expands our current understanding of prokaryotic Sia metabolism within host-associated microbial communities.
- Subjects :
- Bacteria metabolism
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Escherichia coli genetics
Escherichia coli metabolism
N-Acetylneuraminic Acid chemistry
N-Acetylneuraminic Acid analogs & derivatives
Symporters genetics
Symporters metabolism
Organic Anion Transporters
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2080
- Volume :
- 170
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 38488830
- Full Text :
- https://doi.org/10.1099/mic.0.001448