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Antifungal activity of azoles, allylamines, and 8-hidroxiquinolines, alone and in combination, against Malassezia pachydermatis in vitro and in vivo.
- Source :
-
Journal de mycologie medicale [J Mycol Med] 2024 Jun; Vol. 34 (2), pp. 101475. Date of Electronic Publication: 2024 Mar 11. - Publication Year :
- 2024
-
Abstract
- Malassezia pachydermatis is often reported as the causative agent of dermatitis in dogs. This study aims to evaluate the in vitro and in vivo antifungal activity of azoles and terbinafine (TRB), alone and in combination with the 8-hydroxyquinoline derivatives (8-HQs) clioquinol (CQL), 8-hydroxyquinoline-5-(n-4-chlorophenyl)sulfonamide (PH151), and 8-hydroxyquinoline-5-(n-4-methoxyphenyl)sulfonamide (PH153), against 16 M. pachydermatis isolates. Susceptibility to the drugs was evaluated by in vitro broth microdilution and time-kill assays. The Toll-deficient Drosophila melanogaster fly model was used to assess the efficacy of drugs in vivo. In vitro tests showed that ketoconazole (KTZ) was the most active drug, followed by TRB and CQL. The combinations itraconazole (ITZ)+CQL and ITZ+PH151 resulted in the highest percentages of synergism and none of the combinations resulted in antagonism. TRB showed the highest survival rates after seven days of treatment of the flies, followed by CQL and ITZ, whereas the evaluation of fungal burden of dead flies showed a greater fungicidal effect of azoles when compared to the other drugs. Here we showed for the first time that CQL is effective against M. pachydermatis and potentially interesting for the treatment of malasseziosis.<br />Competing Interests: Declaration of competing interest None.<br /> (Copyright © 2024 SFMM. Published by Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Animals
Dogs
Terbinafine pharmacology
Drug Synergism
Drug Therapy, Combination
Dog Diseases microbiology
Dog Diseases drug therapy
Ketoconazole pharmacology
Oxyquinoline pharmacology
Sulfonamides pharmacology
Itraconazole pharmacology
Clioquinol pharmacology
Disease Models, Animal
Antifungal Agents pharmacology
Malassezia drug effects
Malassezia growth & development
Microbial Sensitivity Tests
Azoles pharmacology
Dermatomycoses drug therapy
Dermatomycoses microbiology
Drosophila melanogaster microbiology
Drosophila melanogaster drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1773-0449
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal de mycologie medicale
- Publication Type :
- Academic Journal
- Accession number :
- 38479291
- Full Text :
- https://doi.org/10.1016/j.mycmed.2024.101475