Bone Marrow-Derived C-Kit + Cells Improved Inflammatory IL-33/ST-2/ILC2 Axis in the Lung Tissue of Type 2 Diabetic Rats.

Inflammation is an essential factor in pulmonary complications of diabetes. Bone marrow (BM)-derived C-kit ⁺ cells have immunomodulatory properties and their transplantation is suggested as a promising strategy for ameliorating diabetes complications. This study evaluated the effect of BM-derived C-kit ⁺ cells on the inflammation signaling pathway in lung tissue of type 2 diabetic male rats. Ten rats were used to extract C-kit cells, and 48 male Wistar rats weighing 180 ± 20 g were randomly divided into four equal groups: (1) Control (Cont), (2) Diabetic (D), (3) Diabetic + C-kit ⁺ cells (D + C-kit pos) intravenously injected 50-µl phosphate buffer saline (PBS) containing 300,000 C-kit ⁺ cells, and (4) Diabetic + C-kit ^- cells (D + C-kit neg), intravenously injected C-kit ^- cells. Diabetes induction increased IL-33, ST-2, CD127, and IL-2 levels and decreased IL-10. C-kit ⁺ cell therapy significantly decreased IL-33 and CD127 and increased IL-10. In addition, lung histopathological changes significantly improved in the C-kit ⁺ group compared to the diabetic group. These findings suggest that C-kit ⁺ cells may have a potential therapeutic role in mitigating diabetes-induced respiratory complications via ameliorating the inflammation and histopathological changes in lung tissue.
Competing Interests: Declarations Ethical Approval All study procedures and interventions were conducted according to the Guide for the Care and Use of Laboratory Animals of the National Institute of Health (8th edition, 2011) and approved by the ethics committee of Tabriz University of Medical Sciences (ethical number IR.TBZMED.VCR.REC.1400.141). Consent to Participate Not applicable. Consent for Publication Not applicable. Competing Interests The authors declare no competing interests.
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