Correlations between bone metabolism biomarkers and fluoride exposure in adults and children.

Increased exposure to fluoride, which notably affects bone metabolism, is a global concern. However, the correlations and sensitivity of bone metabolism to fluoride remain controversial. In this cross-sectional study, 549 children (aged 7-12 years) and 504 adults (≥ 18 years old) were recruited in the high-fluoride areas of the Henan Province. Urinary fluoride (UF) level was determined using a fluoride electrode. Fasting venous blood serum was collected to measure bone metabolism biomarkers. The selected bone metabolism biomarkers for children included bone alkaline phosphatase (BALP), serum alkaline phosphatase (ALP), osteocalcin (OCN), calcitonin (CT), parathyroid hormone (PTH), phosphorus (P ⁵⁺ ), and calcium (Ca ²⁺ ). For adults, the biomarkers included ALP, CT, PTH, β-CrossLaps (β-CTX), P ⁵⁺ , and Ca ²⁺ . The correlations between UF and bone metabolism biomarkers were analyzed using binary logistic regression, a trend test, a generalized additive model, and threshold effect analysis. Regression analysis indicated a significant correlation between serum OCN, PTH, and UF levels in children aged 7-9 years. Serum OCN, PTH, and BALP contents were significantly correlated with UF in boys (P < 0.05). Furthermore, the interaction between age and UF affected serum P ⁵⁺ and PTH (P < 0.05). The generalized additive model revealed nonlinear dose-response relationships between P ⁵⁺ , BALP, and UF contents in children (P < 0.05). Serum OCN level was linearly correlated with the UF concentration (P < 0.05). Similarly, a significant correlation was observed between β-CTX and UF levels in adults. In addition, significant correlations were observed between UF-age and serum Ca ²⁺ , β-CTX, and PTH contents. There was a non-linear correlation between serum Ca ²⁺ , P ⁵⁺ , and β- CTX and UF levels (P < 0.05). Overall, serum OCN, BALP, and P ⁵⁺ levels can serve as sensitive bone metabolism biomarkers in children, while β-CTX, P ⁵⁺ , and Ca ²⁺ can be considered fluoride-sensitive bone metabolism biomarkers in adults.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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