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Microfluidic Diffusional Sizing (MDS) Measurements of Secretory Neutralizing Antibody Affinity Against SARS-CoV-2.
- Source :
-
Annals of biomedical engineering [Ann Biomed Eng] 2024 Jun; Vol. 52 (6), pp. 1653-1664. Date of Electronic Publication: 2024 Mar 08. - Publication Year :
- 2024
-
Abstract
- SARS-CoV-2 has rampantly spread around the globe and continues to cause unprecedented loss through ongoing waves of (re)infection. Increasing our understanding of the protection against infection with SARS-CoV-2 is critical to ending the pandemic. Serological assays have been widely used to assess immune responses, but secretory antibodies, the essential first line of defense, have been studied to only a limited extent. Of particular interest and importance are neutralizing antibodies, which block the binding of the spike protein of SARS-CoV-2 to the human receptor angiotensin-converting enzyme-2 (ACE2) and thus are essential for immune defense. Here, we employed Microfluidic Diffusional Sizing (MDS), an immobilization-free technology, to characterize neutralizing antibody affinity to SARS-CoV-2 spike receptor-binding domain (RBD) and spike trimer in saliva. Affinity measurement was obtained through a contrived sample and buffer using recombinant SARS-CoV-2 RBD and monoclonal antibody. Limited saliva samples demonstrated that MDS applies to saliva neutralizing antibody measurement. The ability to disrupt a complex of ACE2-Fc and spike trimer is shown. Using a quantitative assay on the patient sample, we determined the affinity and binding site concentration of the neutralizing antibodies.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
Saliva immunology
Antibody Affinity
Antibodies, Monoclonal immunology
Antibodies, Monoclonal chemistry
Antibodies, Neutralizing immunology
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus immunology
Spike Glycoprotein, Coronavirus chemistry
Angiotensin-Converting Enzyme 2 metabolism
Angiotensin-Converting Enzyme 2 immunology
Angiotensin-Converting Enzyme 2 chemistry
COVID-19 immunology
Antibodies, Viral immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-9686
- Volume :
- 52
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Annals of biomedical engineering
- Publication Type :
- Academic Journal
- Accession number :
- 38459195
- Full Text :
- https://doi.org/10.1007/s10439-024-03478-0