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ATX1 and HUB1/2 promote recruitment of the transcription elongation factor VIP2 to modulate the floral transition in Arabidopsis.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2024 Jun; Vol. 118 (6), pp. 1760-1773. Date of Electronic Publication: 2024 Mar 06. - Publication Year :
- 2024
-
Abstract
- Histone 2B ubiquitination (H2Bub) and trimethylation of H3 at lysine 4 (H3K4me3) are associated with transcription activation. However, the function of these modifications in transcription in plants remains largely unknown. Here, we report that coordination of H2Bub and H3K4me3 deposition with the binding of the RNA polymerase-associated factor VERNALIZATION INDEPENDENCE2 (VIP2) to FLOWERING LOCUS C (FLC) modulates flowering time in Arabidopsis. We found that RING domain protein HISTONE MONOUBIQUITINATION1 (HUB1) and HUB2 (we refer as HUB1/2), which are responsible for H2Bub, interact with ARABIDOPSIS TRITHORAX1 (ATX1), which is required for H3K4me3 deposition, to promote the transcription of FLC and repress the flowering time. The atx1-2 hub1-10 hub2-2 triple mutant in FRIGIDIA (FRI) background displayed early flowering like FRI hub1-10 hub2-2 and overexpression of ATX1 failed to rescue the early flowering phenotype of hub1-10 hub2-2. Mutations in HUB1 and HUB2 reduced the ATX1 enrichment at FLC, indicating that HUB1 and HUB2 are required for ATX1 recruitment and H3K4me3 deposition at FLC. We also found that the VIP2 directly binds to HUB1, HUB2, and ATX1 and that loss of VIP2 in FRI hub1-10 hub2-2 and FRI atx1-2 plants resulted in early flowering like that observed in FRI vip2-10. Loss of function of HUB2 and ATX1 impaired VIP2 enrichment at FLC, and reduced the transcription initiation and elongation of FLC. In addition, mutations in VIP2 reduced HUB1 and ATX1 enrichment and H2Bub and H3K4me3 levels at FLC. Together, our findings revealed that HUB1/2, ATX1, and VIP2 coordinately modulate H2Bub and H3K4me3 deposition, FLC transcription, and flowering time.<br /> (© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
- Subjects :
- Flowers genetics
Flowers physiology
Flowers growth & development
Flowers metabolism
Histone-Lysine N-Methyltransferase genetics
Histone-Lysine N-Methyltransferase metabolism
MADS Domain Proteins genetics
MADS Domain Proteins metabolism
Mutation
Transcription Factors metabolism
Transcription Factors genetics
Transcriptional Elongation Factors metabolism
Transcriptional Elongation Factors genetics
Ubiquitin-Protein Ligases metabolism
Ubiquitin-Protein Ligases genetics
Ubiquitination
Arabidopsis genetics
Arabidopsis physiology
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Arabidopsis Proteins genetics
Gene Expression Regulation, Plant
Histones metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 118
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 38446797
- Full Text :
- https://doi.org/10.1111/tpj.16707