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Tyrosinase from the pulps of local cultivars of Musa spp: Purification, characterization, immobilization, and application in the batch production of l-3,4-dihydroxyphenylalanine.
- Source :
-
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Sep; Vol. 54 (8), pp. 1098-1105. Date of Electronic Publication: 2024 Mar 06. - Publication Year :
- 2024
-
Abstract
- Tyrosinase, an enzyme involved in browning reactions in plants/crops exposed to mechanical injury, was isolated from the pulp of some different locally available bananas ( M. cavendish , M. acuminata , and M. paradisiaca ). Tyrosinase from the pulps was extracted, purified, immobilized, and characterized. Thereafter, the potentials of the immobilized tyrosinase in the possible production of l-3,4-dihydroxyphenylalanine (L-DOPA) in an improvised batch reactor was exploited using tyrosine and ascorbate as the substrates. L-DOPA production was monitored via thin-layer chromatography and spectrophotometry (Arnow's method). L-DOPA is a drug that is used in the treatment of Parkinson's disease. Hence, this study exploited a non-chemical route for its synthesis using the tyrosinase obtained from the banana pulps. The purified tyrosinase had an optimum pH and temperature of 6.5 and 7.0, respectively. The molecular weight of the purified tyrosinase was 45 kDa. Quercetin and resorcinol both competitively inhibited the purified tyrosinase from the three cultivars. Immobilized M. cavendish tyrosinase produced the highest concentration (0.60 mM) of L-DOPA after 8 h in an improvised batch reactor. The tyrosinase in the banana pulps serves as a cheap and readily available green route for the possible production of L-DOPA.
- Subjects :
- Hydrogen-Ion Concentration
Temperature
Monophenol Monooxygenase metabolism
Monophenol Monooxygenase chemistry
Monophenol Monooxygenase isolation & purification
Enzymes, Immobilized chemistry
Enzymes, Immobilized metabolism
Enzymes, Immobilized isolation & purification
Musa chemistry
Levodopa metabolism
Levodopa biosynthesis
Levodopa isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2297
- Volume :
- 54
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Preparative biochemistry & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 38445841
- Full Text :
- https://doi.org/10.1080/10826068.2024.2324084