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Antioxidant and anti-inflammatory potentials of Ammodaucus leucotrichus Coss. & Durieu seeds' extracts: In vitro and in vivo studies.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2024 May 23; Vol. 326, pp. 117964. Date of Electronic Publication: 2024 Feb 23. - Publication Year :
- 2024
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Abstract
- Ethnopharmacological Relevance: Ammodaucus leucotrichus Coss. & Durieu (Apiaceae) is traditionally used in southern Algeria as a remedy against a wide range of disease due to its health-promoting properties.<br />Aim of the Study: To investigate anti-oxidant and anti-inflammatory potentials of plant methanolic extract and its fractions in vitro and in vivo.<br />Materials and Methods: Anti-radical activity was assessed in vitro using ABTS <superscript>•+</superscript> , superoxide anion (O <subscript>2</subscript> <superscript>•-</superscript> ) and nitric oxide radical ( <superscript>•</superscript> NO). Lipid peroxidation inhibition was also investigated in the linoleic acid system. Enzyme inhibition assay was performed against α-amylase and α-glucosidase. The anti-inflammatory effect of extracts was screened in vitro through thermal induction of human serum albumin, and in vivo on a skin acute inflammation model induced by λ-carrageenan paw injection, xylene and croton oil topical application. Analgesic effect was evaluated by acetic acid-induced writhing test.<br />Results: The highest contents of polyphenols and flavonoids was recorded by the crude extract (77.14 ± 0.01 μg GAE/mg E and 19.59 ± 0.08 μg QE/mg E, respectively). Among the extracts, ethyl acetate extract showed a promising anti-radical activity of ABTS <superscript>•+</superscript> , O <subscript>2</subscript> <superscript>•-</superscript> and <superscript>•</superscript> NO, in addition to a remarkable inhibition activity of the tested enzymes. Meanwhile, all extracts effectively protected linoleic acid against lipid peroxidation and human serum albumin structure in thermal condition even at low concentration (0.31 mg/ml). Oral administration of 200 mg/kg of crude extract successfully inhibited acetic acid induced nociception and reduced edema formation induced by xylene and carrageenan. However, a dose-dependent manner was observed to decrease ear edema by a microscopic examination in croton oil induced acute inflammation. Nitrite and malondialdehyde levels together with catalase activity were modulated in the presence of plant-derived bioactive compounds.<br />Conclusions: This study showed that Ammodaucus leucotrichus is potentially rich source of anti-oxidant and anti-inflammatory bioactive compounds.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Croton Oil
Linoleic Acid
Phytotherapy
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents therapeutic use
Plant Extracts pharmacology
Plant Extracts therapeutic use
Carrageenan
Acetic Acid therapeutic use
Inflammation
Edema chemically induced
Edema drug therapy
Seeds
Serum Albumin, Human
Analgesics pharmacology
Antioxidants pharmacology
Antioxidants therapeutic use
Antioxidants chemistry
Xylenes
Sulfonic Acids
Benzothiazoles
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 326
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 38401663
- Full Text :
- https://doi.org/10.1016/j.jep.2024.117964