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Development of an ELISA for the detection of fowl adenovirus serotype -4 utilizing fiber protein.

Authors :
Ather F
Zia MA
Habib M
Shah MS
Source :
Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2024 Feb; Vol. 85, pp. 101752. Date of Electronic Publication: 2024 Feb 23.
Publication Year :
2024

Abstract

Hydropericardium syndrome (HPS), caused by the Fowl adenovirus 4 (FAdV-4) has led to significant financial losses for the poultry industry globally, including Pakistan over the past few years. Conventional serological methods are time consuming, laborious and less sensitive therefore, a rapid and sensitive ELISA kit is required for the reliable detection of FAdV-4 infection. In the current research, fiber proteins (1 &2) of FAdV-4 were successfully expressed in Escherichia coli and purified using metal affinity chromatography. Using these proteins as antigens, an indirect ELISA for detecting FAdV-4 infection was developed. The developed ELISA showed superior performances upon comparison with Serum neutralization test (SNT). This ELISA also showed reliable detection of FAdV specific antibodies in experimentally infected and vaccinated chickens. This assay produced good correlation on the samples collected from the field with SNT and found essential for large scale serology of the FAdV. No cross reactivity was observed in the ELISA following the testing of the serum samples of different other avian pathogens which showed that this ELISA is specific in detecting the FAdV infection. In conclusion, the developed Fiber protein ELISA is highly sensitive and specific in the detecting the FAdV infection and can be utilized for large scale sero-epidemiology of the disease.<br /> (Copyright © 2024 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1095-8320
Volume :
85
Database :
MEDLINE
Journal :
Biologicals : journal of the International Association of Biological Standardization
Publication Type :
Academic Journal
Accession number :
38401400
Full Text :
https://doi.org/10.1016/j.biologicals.2024.101752