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Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest Paralipsa gularis (Zeller).
- Source :
-
Toxins [Toxins (Basel)] 2024 Feb 07; Vol. 16 (2). Date of Electronic Publication: 2024 Feb 07. - Publication Year :
- 2024
-
Abstract
- Paralipsa gularis (Zeller) is a storage pest; however, in recent years it has evolved into a considerable maize pest during the late growth stage in the border region between China and other Southeast Asian countries. Bt transgenic insect-resistant maize is an effective measure in controlling a wide range of lepidopteran pests, but there is a lack of research on the toxic effects of storage pests. We tested the toxicity of Bt-Cry1Ab, Vip3Aa, and their complex proteins against P. gularis via bioassay and investigated the efficiency of Bt-(Cry1Ab+Vip3Aa) maize in controlling P. gularis during the late growth stage of maize in the period 2022-2023. The bioassay results show that the susceptibilities of P. gularis to the two Bt proteins and their complex proteins were significantly different. The LC <subscript>50</subscript> values of DBNCry1Ab ("DBN9936" event), DBNVip3Aa ("DBN9501" event), DBN Cry1Ab+Vip3Aa ("DBN3601T" event), and Syngenta Cry1Ab+Vip3Aa ("Bt11" event × "MIR162" event) were 0.038 μg/g, 0.114 μg/g, 0.110 μg/g, and 0.147 μg/g, and the GIC <subscript>50</subscript> values were 0.014 μg/g, 0.073 μg/g, 0.027 μg/g, and 0.026 μg/g, respectively. Determination of the expression content of the insecticidal protein in different tissues of Bt-(Cry1Ab+Vip3Aa) maize shows that the total Bt protein content in different tissues was in the following order: stalk > bract > cob > kernel. However, the bioassay results show that the mortalities of P. gularis feeding on Bt-(Cry1Ab+Vip3Aa) maize in different tissues at different growth stages were all above 93.00%. The field trial indicates that the occurrence density of larvae and plant damage rate for conventional maize were 422.10 individuals/100 plants and 94.40%, respectively, whereas no larvae were found on Bt-(Cry1Ab+Vip3Aa) maize. In summary, this study implies that Bt-(Cry1Ab+Vip3Aa) maize has a high potential for control of P. gularis , providing a new technical measure for the management of the pest.
- Subjects :
- Humans
Animals
Zea mays genetics
Zea mays metabolism
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Endotoxins metabolism
Bacillus thuringiensis Toxins metabolism
Bacterial Proteins toxicity
Bacterial Proteins genetics
Hemolysin Proteins toxicity
Hemolysin Proteins genetics
Pest Control, Biological methods
Larva
Bacillus thuringiensis genetics
Bacillus thuringiensis metabolism
Lepidoptera metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 38393170
- Full Text :
- https://doi.org/10.3390/toxins16020092