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Synchronous force and Ca 2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium.

Authors :
Sun Z
Lu K
Kamla C
Kameritsch P
Seidel T
Dendorfer A
Source :
Communications biology [Commun Biol] 2024 Feb 22; Vol. 7 (1), pp. 220. Date of Electronic Publication: 2024 Feb 22.
Publication Year :
2024

Abstract

Dysfunctional Ca <superscript>2+</superscript> signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca <superscript>2+</superscript> and force is essential to investigate the relationship between contractility and Ca <superscript>2+</superscript> signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca <superscript>2+</superscript> and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca <superscript>2+</superscript> were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca <superscript>2+</superscript> signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.<br /> (© 2024. The Author(s).)

Details

Language :
English
ISSN :
2399-3642
Volume :
7
Issue :
1
Database :
MEDLINE
Journal :
Communications biology
Publication Type :
Academic Journal
Accession number :
38388802
Full Text :
https://doi.org/10.1038/s42003-024-05886-3