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Synchronous force and Ca 2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium.
- Source :
-
Communications biology [Commun Biol] 2024 Feb 22; Vol. 7 (1), pp. 220. Date of Electronic Publication: 2024 Feb 22. - Publication Year :
- 2024
-
Abstract
- Dysfunctional Ca <superscript>2+</superscript> signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca <superscript>2+</superscript> and force is essential to investigate the relationship between contractility and Ca <superscript>2+</superscript> signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca <superscript>2+</superscript> and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca <superscript>2+</superscript> were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca <superscript>2+</superscript> signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.<br /> (© 2024. The Author(s).)
Details
- Language :
- English
- ISSN :
- 2399-3642
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Communications biology
- Publication Type :
- Academic Journal
- Accession number :
- 38388802
- Full Text :
- https://doi.org/10.1038/s42003-024-05886-3