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Temporal-Specific Sex and Injury-Dependent Changes on Neurogranin-Associated Synaptic Signaling After Controlled Cortical Impact in Rats.
- Source :
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Molecular neurobiology [Mol Neurobiol] 2024 Sep; Vol. 61 (9), pp. 7256-7268. Date of Electronic Publication: 2024 Feb 20. - Publication Year :
- 2024
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Abstract
- Extensive effort has been made to study the role of synaptic deficits in cognitive impairment after traumatic brain injury (TBI). Neurogranin (Ng) is a calcium-sensitive calmodulin (CaM)-binding protein essential for Ca <superscript>2+</superscript> /CaM-dependent kinase II (CaMKII) autophosphorylation which subsequently modulates synaptic plasticity. Given the loss of Ng expression after injury, additional research is warranted to discern changes in hippocampal post-synaptic signaling after TBI. Under isoflurane anesthesia, adult, male and female Sprague-Dawley rats received a sham/control or controlled cortical impact (CCI) injury. Ipsilateral hippocampal synaptosomes were isolated at 24 h and 1, 2, and 4 weeks post-injury, and western blot was used to evaluate protein expression of Ng-associated signaling proteins. Non-parametric Mann-Whitney tests were used to determine significance of injury for each sex at each time point. There were significant changes in the hippocampal synaptic expression of Ng and associated synaptic proteins such as phosphorylated Ng, CaMKII, and CaM up to 4 weeks post-CCI, demonstrating TBI alters hippocampal post-synaptic signaling. This study furthers our understanding of mechanisms of cognitive dysfunction within the synapse sub-acutely after TBI.<br /> (© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
- Subjects :
- Animals
Female
Male
Rats
Calmodulin metabolism
Cerebral Cortex metabolism
Cerebral Cortex pathology
Phosphorylation
Sex Characteristics
Synaptosomes metabolism
Time Factors
Brain Injuries, Traumatic metabolism
Brain Injuries, Traumatic pathology
Brain Injuries, Traumatic complications
Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism
Hippocampus metabolism
Hippocampus pathology
Neurogranin metabolism
Rats, Sprague-Dawley
Signal Transduction physiology
Synapses metabolism
Synapses pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1182
- Volume :
- 61
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 38376763
- Full Text :
- https://doi.org/10.1007/s12035-024-04043-5