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Time-kill kinetic of nano-ZnO-loaded nanoliposomes against Aspergillus niger and Botrytis cinerea.
- Source :
-
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1669-1678. Date of Electronic Publication: 2024 Feb 19. - Publication Year :
- 2024
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Abstract
- In vitro antimicrobial activity of nano-ZnO-loaded nanoliposomes at different levels of lecithin:nano-ZnO ratio (5:1, 15:1, and 25:1 w/w) against Aspergillus niger (IBRC-M 30095) and Botrytis cinerea (IBRC-M 30162) was evaluated. Nanoliposome formulations containing nano-ZnO were fabricated through thin-layer hydration sonication and heat methods. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of nano-ZnO-loaded nanoliposomes and free nano-ZnO against Aspergillus niger and Botrytis cinerea were determined. The time-kill experiments were performed for each isolate. Results showed that the encapsulation of nano-ZnO in nanoliposome systems significantly enhanced their antimicrobial activities by improving the penetration of ZnO nanoparticles the fungi cell membrane. In vitro antifungal activity of nano-ZnO-loaded nanoliposomes against Aspergillus niger and Botrytis cinerea was increased in thin-layer hydration sonication method compared with the heat method. The log phase for Aspergillus niger and Botrytis cinerea was around 70 h. Adding nano-ZnO-loaded nanoliposomes to the culture medium shortened the log phase for both Aspergillus niger and Botrytis cinerea. The highest antimicrobial activity of nanoliposomes was achieved using nanoliposomes containing the lecithin:nano-ZnO ratio of 25:1 (w/w) as compared to all samples. However, the length of the log phase growth cultures exposed to the nanoliposome formulations prepared by thin-layer hydration sonication method with the lecithin:nano-ZnO ratio of 25:1 (w/w) at MIC and MFC values was 60 and 40 h for both Aspergillus niger and Botrytis cinerea, respectively.<br /> (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
- Subjects :
- Kinetics
Nanoparticles chemistry
Aspergillus niger drug effects
Aspergillus niger growth & development
Botrytis drug effects
Botrytis growth & development
Zinc Oxide pharmacology
Zinc Oxide chemistry
Liposomes chemistry
Microbial Sensitivity Tests
Antifungal Agents pharmacology
Antifungal Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1678-4405
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Publication Type :
- Academic Journal
- Accession number :
- 38369671
- Full Text :
- https://doi.org/10.1007/s42770-024-01273-6