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Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland.
- Source :
-
Scientific reports [Sci Rep] 2024 Feb 16; Vol. 14 (1), pp. 3877. Date of Electronic Publication: 2024 Feb 16. - Publication Year :
- 2024
-
Abstract
- Knowing about the antibiotic resistance, serotypes, and virulence-associated genes of Group B Streptococcus for epidemiological and vaccine development is very important. We have determined antimicrobial susceptibility patterns, serotype, and virulence profiles. The antibiotic susceptibility was assessed for a total of 421 Streptococcus agalactiae strains, isolated from pregnant women and neonates. Then, 89 erythromycin and/or clindamycin-resistant strains (82 isolates obtained from pregnant women and seven isolates derived from neonates) were assessed in detail. PCR techniques were used to identify the studied strains, perform serotyping, and assess genes encoding selected virulence factors. Phenotypic and genotypic methods determined the mechanisms of resistance. All tested strains were sensitive to penicillin and levofloxacin. The constitutive MLS <subscript>B</subscript> mechanism (78.2%), inducible MLS <subscript>B</subscript> mechanism (14.9%), and M phenotype (6.9%) were identified in the macrolide-resistant strains. It was found that macrolide resistance is strongly associated with the presence of the ermB gene and serotype V. FbsA, fbsB, fbsC, scpB, and lmb formed the most recurring pattern of genes among the nine surface proteins whose genes were analysed. A minority (7.9%) of the GBS isolates exhibited resistance to lincosamides and macrolides, or either, including those that comprised the hypervirulent clone ST-17. The representative antibiotic resistance pattern consisted of erythromycin, clindamycin, and tetracycline resistance (71.9%). An increase in the fraction of strains resistant to macrolides and lincosamides indicates the need for monitoring both the susceptibility of these strains and the presence of the ST-17 clone.<br /> (© 2024. The Author(s).)
- Subjects :
- Infant, Newborn
Female
Humans
Pregnancy
Macrolides pharmacology
Streptococcus agalactiae
Clindamycin pharmacology
Pregnant Women
Poland epidemiology
Drug Resistance, Bacterial genetics
Microbial Sensitivity Tests
Lincosamides pharmacology
Erythromycin pharmacology
Anti-Bacterial Agents pharmacology
Streptococcal Infections drug therapy
Streptococcal Infections epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 38366099
- Full Text :
- https://doi.org/10.1038/s41598-024-54521-y