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Mechanism of LncRNA FTX regulates nephroblastoma progression through MiR-215-5p/PI3K/AKT axis.
- Source :
-
Journal of pediatric urology [J Pediatr Urol] 2024 Jun; Vol. 20 (3), pp. 491.e1-491.e8. Date of Electronic Publication: 2024 Jan 28. - Publication Year :
- 2024
-
Abstract
- Background: Nephroblastoma, also more commonly known as Wilms tumor (WT), is a common childhood malignancy that connects tumorigenesis and organ development in the kidney.<br />Objective: The current study focused on the effect of lncRNA FTX in nephroblastoma.<br />Study Design: Expression of lncRNA FTX in nephroblastoma tissues and cells was determined. The expression location of lncRNA FTX was detected by FISH. The binding of lncRNA FTX and miR-215-5p with Ago2 was verified by RIP. Following gain- and loss-of-function approaches, the crucial role of lncRNA FTX and miR-215-5p in nephroblastoma cell functions was measured with the involvement of the PI3K/AKT pathway.<br />Results: LncRNA FTX was elevated and miR-215-5p was declined in nephroblastoma. Silencing of lncRNA FTX or mimic of miR-215-5p inhibited the malignant properties of nephroblastoma cells. LncRNA FTX was localized in the cytoplasm and might bind miR-215-5p. LncRNA FTX promoted the malignant features of nephroblastoma cells by inhibiting miR-215-5p through activating of the PI3K/AKT pathway.<br />Conclusions: LncRNA FTX is capable of accelerating nephroblastoma development in vitro by reducing miR-215-5p through activating of the PI3K/AKT pathway, indicating LncRNA FTX may possibly a future target for the diagnosis and treatment of nephroblastoma. SUMMARY FIGURE.<br />Competing Interests: Conflict of interest Authors declare no conflict of interests.<br /> (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Humans
Cell Proliferation
Gene Expression Regulation, Neoplastic
Signal Transduction
Tumor Cells, Cultured
Disease Progression
Kidney Neoplasms genetics
Kidney Neoplasms pathology
Kidney Neoplasms metabolism
MicroRNAs genetics
MicroRNAs metabolism
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Wilms Tumor genetics
Wilms Tumor pathology
Wilms Tumor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4898
- Volume :
- 20
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of pediatric urology
- Publication Type :
- Academic Journal
- Accession number :
- 38365477
- Full Text :
- https://doi.org/10.1016/j.jpurol.2024.01.023