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Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus.

Authors :
Nielsen AKR
Lilieholm-Røngren L
Schmid B
Holst B
Brodin B
Saaby L
Source :
Stem cell research [Stem Cell Res] 2024 Apr; Vol. 76, pp. 103348. Date of Electronic Publication: 2024 Feb 13.
Publication Year :
2024

Abstract

The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1876-7753
Volume :
76
Database :
MEDLINE
Journal :
Stem cell research
Publication Type :
Academic Journal
Accession number :
38364505
Full Text :
https://doi.org/10.1016/j.scr.2024.103348