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Deletion of Kallikrein-related peptidases ( Klks ) has no effect on fertility in mice.
- Source :
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MicroPublication biology [MicroPubl Biol] 2024 Jan 19; Vol. 2024. Date of Electronic Publication: 2024 Jan 19 (Print Publication: 2024). - Publication Year :
- 2024
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Abstract
- Kallikreins (KLKs) are serine peptidases. It was established that Klks are estrogen-target genes in mouse uteri. However, the functional requirement of KLK family in the uterine function during reproduction is unknown. Here we generated a compound deletion of Klk1b3, Klk1b4, Klk1b5, and Klk1 in a mouse model using CRISPR/Cas9 strategy with four single guide RNAs (sgRNAs) to target the second exon of these four genes that are aligned back-to-back in a single locus spanning 32.95 kb on chromosome 7. We found that both male and female knockout mice are fertile with no apparent health defect compared to wild-type controls. Our data suggest that Klk1b3, Klk1b4, Klk1b5, and Klk1 are not necessary for male and female reproductive function in mice.<br />Competing Interests: The authors declare that there are no conflicts of interest present.<br /> (Copyright: © 2024 by the authors.)
Details
- Language :
- English
- ISSN :
- 2578-9430
- Volume :
- 2024
- Database :
- MEDLINE
- Journal :
- MicroPublication biology
- Publication Type :
- Academic Journal
- Accession number :
- 38344070
- Full Text :
- https://doi.org/10.17912/micropub.biology.001070