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Photonic control of ligand nanospacing in self-assembly regulates stem cell fate.
- Source :
-
Bioactive materials [Bioact Mater] 2023 Dec 26; Vol. 34, pp. 164-180. Date of Electronic Publication: 2023 Dec 26 (Print Publication: 2024). - Publication Year :
- 2023
-
Abstract
- Extracellular matrix (ECM) undergoes dynamic inflation that dynamically changes ligand nanospacing but has not been explored. Here we utilize ECM-mimicking photocontrolled supramolecular ligand-tunable Azo <superscript>+</superscript> self-assembly composed of azobenzene derivatives (Azo <superscript>+</superscript> ) stacked via cation-π interactions and stabilized with RGD ligand-bearing poly(acrylic acid). Near-infrared-upconverted-ultraviolet light induces cis -Azo <superscript>+</superscript> -mediated inflation that suppresses cation-π interactions, thereby inflating liganded self-assembly. This inflation increases nanospacing of "closely nanospaced" ligands from 1.8 nm to 2.6 nm and the surface area of liganded self-assembly that facilitate stem cell adhesion, mechanosensing, and differentiation both in vitro and in vivo , including the release of loaded molecules by destabilizing water bridges and hydrogen bonds between the Azo <superscript>+</superscript> molecules and loaded molecules. Conversely, visible light induces trans -Azo <superscript>+</superscript> formation that facilitates cation-π interactions, thereby deflating self-assembly with "closely nanospaced" ligands that inhibits stem cell adhesion, mechanosensing, and differentiation. In stark contrast, when ligand nanospacing increases from 8.7 nm to 12.2 nm via the inflation of self-assembly, the surface area of "distantly nanospaced" ligands increases, thereby suppressing stem cell adhesion, mechanosensing, and differentiation. Long-term in vivo stability of self-assembly via real-time tracking and upconversion are verified. This tuning of ligand nanospacing can unravel dynamic ligand-cell interactions for stem cell-regulated tissue regeneration.<br />Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (© 2023 The Authors.)
Details
- Language :
- English
- ISSN :
- 2452-199X
- Volume :
- 34
- Database :
- MEDLINE
- Journal :
- Bioactive materials
- Publication Type :
- Academic Journal
- Accession number :
- 38343773
- Full Text :
- https://doi.org/10.1016/j.bioactmat.2023.12.011