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Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning.
- Source :
-
Nature plants [Nat Plants] 2024 Mar; Vol. 10 (3), pp. 423-438. Date of Electronic Publication: 2024 Feb 09. - Publication Year :
- 2024
-
Abstract
- Centromeres strongly affect (epi)genomic architecture and meiotic recombination dynamics, influencing the overall distribution and frequency of crossovers. Here we show how recombination is regulated and distributed in the holocentric plant Rhynchospora breviuscula, a species with diffused centromeres. Combining immunocytochemistry, chromatin analysis and high-throughput single-pollen sequencing, we discovered that crossover frequency is distally biased, in sharp contrast to the diffused distribution of hundreds of centromeric units and (epi)genomic features. Remarkably, we found that crossovers were abolished inside centromeric units but not in their proximity, indicating the absence of a canonical centromere effect. We further propose that telomere-led synapsis of homologues is the feature that best explains the observed recombination landscape. Our results hint at the primary influence of mechanistic features of meiotic pairing and synapsis rather than (epi)genomic features and centromere organization in determining the distally biased crossover distribution in R. breviuscula, whereas centromeres and (epi)genetic properties only affect crossover positioning locally.<br /> (© 2024. The Author(s).)
- Subjects :
- Centromere genetics
Homologous Recombination
Chromosome Pairing
Subjects
Details
- Language :
- English
- ISSN :
- 2055-0278
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nature plants
- Publication Type :
- Academic Journal
- Accession number :
- 38337039
- Full Text :
- https://doi.org/10.1038/s41477-024-01625-y