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Protein kinase D drives the secretion of invasion mediators in triple-negative breast cancer cell lines.
- Source :
-
IScience [iScience] 2024 Jan 17; Vol. 27 (2), pp. 108958. Date of Electronic Publication: 2024 Jan 17 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- The protein kinase D (PKD) family members regulate the fission of cargo vesicles at the Golgi complex and play a pro-oncogenic role in triple-negative breast cancer (TNBC). Whether PKD facilitates the secretion of tumor-promoting factors in TNBC, however, is still unknown. Using the pharmacological inhibition of PKD activity and siRNA-mediated depletion of PKD2 and PKD3, we identified the PKD-dependent secretome of the TNBC cell lines MDA-MB-231 and MDA-MB-468. Mass spectrometry-based proteomics and antibody-based assays revealed a significant downregulation of extracellular matrix related proteins and pro-invasive factors such as LIF, MMP-1, MMP-13, IL-11, M-CSF and GM-CSF in PKD-perturbed cells. Notably, secretion of these proteins in MDA-MB-231 cells was predominantly controlled by PKD2 and enhanced spheroid invasion. Consistently, PKD-dependent secretion of pro-invasive factors was more pronounced in metastatic TNBC cell lines. Our study thus uncovers a novel role of PKD2 in releasing a pro-invasive secretome.<br />Competing Interests: The authors declare no competing interests.<br /> (© 2024 The Authors.)
Details
- Language :
- English
- ISSN :
- 2589-0042
- Volume :
- 27
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- IScience
- Publication Type :
- Academic Journal
- Accession number :
- 38323010
- Full Text :
- https://doi.org/10.1016/j.isci.2024.108958