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Paternò-Büchi Reaction Mass Spectrometry Enables Positional Assignment of Polymethylene-Interrupted Double Bonds in Food-Derived Lipids.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Feb 14; Vol. 72 (6), pp. 3180-3188. Date of Electronic Publication: 2024 Feb 03. - Publication Year :
- 2024
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Abstract
- Fatty acids (FAs) containing polymethylene-interrupted (PMI) double bonds are a component of human foods; however, they present a significant analytical challenge for de novo identification. Covalent adduct chemical ionization and ozone-induced dissociation mass spectrometry (MS) methods enable unambiguous assignment of PMI-FA double bond positions, however, no method has been reported with electrospray ionization (ESI) platform using off-the-shelf systems. In the current work, we studied the Paternò-Büchi (PB) fragmentation patterns of PMI-FA and triacylglycerol (TG) by analyzing several known food sources. PB-MS/MS and MS <superscript>3</superscript> enabled complete double bond location assignments, including the isolated double bond in PMI-FA and triacylglycerols. Sea urchin ("uni"), oyster, pine nut, and ginkgo nut were characterized for their signature PMI-FA, 20:2(5 Z ,11 Z ), 22:2(7 Z , 15 Z ), 18:3(5 Z ,9 Z ,12 Z ), and 20:3(5 Z ,11 Z ,14 Z ), respectively. Quantitative analyses of the relative abundance of these PMI-FA led to results similar to reference methods. 18:3(5 Z ,9 Z ,12 Z ) was enriched at the sn -1/ sn -3 position in pine nut major TG.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38308634
- Full Text :
- https://doi.org/10.1021/acs.jafc.3c06366