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Anti-inflammatory effects of reticuline on the JAK2/STAT3/SOCS3 and p38 MAPK/NF-κB signaling pathway in a mouse model of obesity-associated asthma.
- Source :
-
The clinical respiratory journal [Clin Respir J] 2024 Jan; Vol. 18 (1), pp. e13729. - Publication Year :
- 2024
-
Abstract
- Background: Asthma associated with obesity is a chronic disease characterized by earlier airway remodeling, severe wheezing, and increased insensitivity to hormone therapy. Reticuline, a bioactive compound of Magnoliae Flos, exerts anti-inflammatory activity and can inhibit neutrophil recruitment. Thus, this study investigated the role of reticuline in obesity-related asthma.<br />Methods: The BALB/c mice fed a low-fat diet (LFD) and high-fat diet (HFD) were intranasally challenged with house dust mites (HDMs) or ovalbumin (OVA). Reticuline (0.25 mg/kg) was administrated into mice by intragastrical gavage. Airway hyper-responsiveness was examined after the final challenge. Body weight was measured, and bronchoalveolar lavage fluid (BALF) and lung tissues were collected. The number of inflammatory cells in BALF was estimated. Histological changes were assessed by performing hematoxylin-eosin staining, and production of proinflammatory cytokines and IgE was examined by ELISA kits. Related pathways were studied with western blotting.<br />Results: Reticuline suppressed airway resistance and inflammatory infiltration in lung tissue and reduced inflammatory cell recruitment in BALF in obesity mice with asthma. Additionally, the levels of IL-17A, IL-1β, IL-5, macrophage inflammatory protein 2, and regulated on activation, normal T cell expressed and secreted in the lung were reduced by reticuline. Mechanistically, reticuline inactivated the JAK2/STAT3/SOCS3 and p38 MAPK/NF-κB signaling pathways in obesity-related asthma.<br />Conclusion: Reticuline alleviates airway inflammation in obesity-related asthma by inactivating the JAK2/STAT3/SOCS3 and p38 MAPK/NF-κB signaling pathways.<br /> (© 2024 The Authors. The Clinical Respiratory Journal published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Mice
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents therapeutic use
Bronchoalveolar Lavage Fluid
Cytokines metabolism
Disease Models, Animal
Inflammation metabolism
Lung pathology
Mice, Inbred BALB C
Obesity complications
Obesity drug therapy
p38 Mitogen-Activated Protein Kinases metabolism
p38 Mitogen-Activated Protein Kinases pharmacology
p38 Mitogen-Activated Protein Kinases therapeutic use
Signal Transduction
Suppressor of Cytokine Signaling 3 Protein drug effects
Suppressor of Cytokine Signaling 3 Protein metabolism
Asthma drug therapy
Asthma metabolism
Benzylisoquinolines pharmacology
Benzylisoquinolines therapeutic use
Janus Kinase 2 drug effects
Janus Kinase 2 metabolism
NF-kappa B drug effects
NF-kappa B metabolism
STAT3 Transcription Factor drug effects
STAT3 Transcription Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1752-699X
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The clinical respiratory journal
- Publication Type :
- Academic Journal
- Accession number :
- 38286741
- Full Text :
- https://doi.org/10.1111/crj.13729