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Neuropathological Applications of Microscopy with Ultraviolet Surface Excitation (MUSE): A Concordance Study of Human Primary and Metastatic Brain Tumors.

Authors :
Lechpammer M
Todd A
Tang V
Morningstar T
Borowsky A
Shahlaie K
Kintner JA
McPherson JD
Bishop JW
Fereidouni F
Harmany ZT
Coley N
Zagzag D
Wong JWH
Tao J
Hesson LB
Burnett L
Levenson R
Source :
Brain sciences [Brain Sci] 2024 Jan 22; Vol. 14 (1). Date of Electronic Publication: 2024 Jan 22.
Publication Year :
2024

Abstract

Whereas traditional histology and light microscopy require multiple steps of formalin fixation, paraffin embedding, and sectioning to generate images for pathologic diagnosis, Microscopy using Ultraviolet Surface Excitation (MUSE) operates through UV excitation on the cut surface of tissue, generating images of high resolution without the need to fix or section tissue and allowing for potential use for downstream molecular tests. Here, we present the first study of the use and suitability of MUSE microscopy for neuropathological samples. MUSE images were generated from surgical biopsy samples of primary and metastatic brain tumor biopsy samples (n = 27), and blinded assessments of diagnoses, tumor grades, and cellular features were compared to corresponding hematoxylin and eosin (H&E) images. A set of MUSE-treated samples subsequently underwent exome and targeted sequencing, and quality metrics were compared to those from fresh frozen specimens. Diagnostic accuracy was relatively high, and DNA and RNA integrity appeared to be preserved for this cohort. This suggests that MUSE may be a reliable method of generating high-quality diagnostic-grade histologic images for neuropathology on a rapid and sample-sparing basis and for subsequent molecular analysis of DNA and RNA.<br />Competing Interests: M.L. was employed by the Foundation Medicine, Inc., R.L. is a co-founder of start-up MUSE Microscopy Inc. (now part of SmartPath Dx), and R.L., F.F. and A.B. are co-founders of start-up Histolix Inc. MUSE Microscopy Inc. and Histolix Inc both had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results. The other authors declare no conflicts of interest.

Details

Language :
English
ISSN :
2076-3425
Volume :
14
Issue :
1
Database :
MEDLINE
Journal :
Brain sciences
Publication Type :
Academic Journal
Accession number :
38275528
Full Text :
https://doi.org/10.3390/brainsci14010108