Cholecalciferol Exhibits no Antibacterial Effect on Staphylococcus aureus and Escherichia coli : An in vitro Study.

Background: The pleiotropic effect of cholecalciferol (vitamin D ₃ ) has gained significant momentum and has been explored widely.
Objectives: The study aimed to investigate the antimicrobial effect of cholecalciferol against S. aureus and E. coli .
Methods: An in vitro study was performed for the antimicrobial effect of cholecalciferol against S. aureus and E. coli . The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined following the broth microdilution method.
Results: The MIC value of cholecalciferol against both S. aureus and E. coli was 0.312 mg/ml, and the MBC for both organisms was 1.25 mg/ml. However, we also observed a significant antimicrobial effect in the dimethyl sulfoxide (DMSO) control at 12.5% (v/v). Therefore, the observed antimicrobial effect may be attributed to DMSO, indicating cholecalciferol does not directly inhibit S. aureus and E. coli .
Conclusion: This study indicates that cholecalciferol does not directly inhibit S. aureus and E. coli. Hence, we suggest exploring the antibacterial properties of other vitamin D analogs, such as calcitriol or its synergetic effect with other antimicrobial agents.
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