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Genomic overview of INA-induced NPR1 targeting and transcriptional cascades in Arabidopsis.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2024 Apr 24; Vol. 52 (7), pp. 3572-3588. - Publication Year :
- 2024
-
Abstract
- The phytohormone salicylic acid (SA) triggers transcriptional reprogramming that leads to SA-induced immunity in plants. NPR1 is an SA receptor and master transcriptional regulator in SA-triggered transcriptional reprogramming. Despite the indispensable role of NPR1, genome-wide direct targets of NPR1 specific to SA signaling have not been identified. Here, we report INA (functional SA analog)-specific genome-wide targets of Arabidopsis NPR1 in plants expressing GFP-fused NPR1 under its native promoter. Analyses of NPR1-dependently expressed direct NPR1 targets revealed that NPR1 primarily activates genes encoding transcription factors upon INA treatment, triggering transcriptional cascades required for INA-induced transcriptional reprogramming and immunity. We identified genome-wide targets of a histone acetyltransferase, HAC1, including hundreds of co-targets shared with NPR1, and showed that NPR1 and HAC1 regulate INA-induced histone acetylation and expression of a subset of the co-targets. Genomic NPR1 targeting was principally mediated by TGACG-motif binding protein (TGA) transcription factors. Furthermore, a group of NPR1 targets mostly encoding transcriptional regulators was already bound to NPR1 in the basal state and showed more rapid and robust induction than other NPR1 targets upon SA signaling. Thus, our study unveils genome-wide NPR1 targeting, its role in transcriptional reprogramming, and the cooperativity between NPR1, HAC1, and TGAs in INA-induced immunity.<br /> (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Histones metabolism
Histones genetics
Transcription Factors metabolism
Transcription Factors genetics
Transcription, Genetic drug effects
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors metabolism
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors genetics
Histone Acetyltransferases metabolism
Histone Acetyltransferases genetics
Acetylation
Signal Transduction genetics
Promoter Regions, Genetic
Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Gene Expression Regulation, Plant
Genome, Plant
Salicylic Acid pharmacology
Salicylic Acid metabolism
Arsenate Reductases
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 52
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 38261978
- Full Text :
- https://doi.org/10.1093/nar/gkae019