Self-assembly of Copper Nanoclusters Using DNA Nanoribbon Templates for Sensitive Electrochemical Detection of H 2 O 2 in Live Cells.

The excessive secretion of H ₂ O ₂ within cells is closely associated with cellular dysfunction. Therefore, high sensitivity in situ detection of H ₂ O ₂ released from living cells was valuable in clinical diagnosis. In the present work, a novel electrochemical cells sensing platform by synthesizing copper nanoclusters (CuNCs) at room temperature based on DNA nanoribbon (DNR) as a template (DNR-CuNCs). The tight and ordered arrangement of nanostructured assemblies of DNR-CuNCs conferred the sensor with superior stability (45 days) and electrochemical performance. The MUC1 aptamer extending from the DNR template enabled the direct capture MCF-7 cells on electrode surface, this facilitated real-time monitoring of H ₂ O ₂ release from stimulated MCF-7 cells. While the captured MCF-7 cells on the electrode surface significantly amplified the current signal of H ₂ O ₂ release compared with the traditional electrochemical detection H ₂ O ₂ released signal by MCF-7 cells in PBS solution. The approach provides an effective strategy for the design of versatile sensors and achieving monitored cell release of H ₂ O ₂ in long time horizon (10 h). Thereby expanding the possibilities for detecting biomolecules from live cells in clinical diagnosis and biomedical applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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