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Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation.
- Source :
-
Immunity [Immunity] 2024 Feb 13; Vol. 57 (2), pp. 245-255.e5. Date of Electronic Publication: 2024 Jan 15. - Publication Year :
- 2024
-
Abstract
- Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. High-affinity cells are proposed to preferentially select into this compartment, potentiating the immune response. We used single-cell RNA-seq to track the germinal center (GC) development of Igh <superscript>g2A10</superscript> B cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites, we identified 3 populations of cells in the GC light zone (LZ). One LZ population expressed a gene signature associated with the initiation of PC differentiation and readily formed PCs in vitro. The estimated affinity of these pre-PC B cells was indistinguishable from that of LZ cells that remained in the GC. This remained true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. These findings suggest that the initiation of PC development occurs via an affinity-independent process.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1097-4180
- Volume :
- 57
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 38228150
- Full Text :
- https://doi.org/10.1016/j.immuni.2023.12.010