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Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols: A Guideline for Regenerative Medicine.

Authors :
Stricker PEF
de Oliveira NB
Mogharbel BF
Lührs L
Irioda AC
Abdelwahid E
Regina Cavalli L
Zotarelli-Filho IJ
de Carvalho KAT
Source :
Current stem cell research & therapy [Curr Stem Cell Res Ther] 2024; Vol. 19 (7), pp. 1009-1020.
Publication Year :
2024

Abstract

Background: This systematic review describes the most common methodologies for immortalizing human and animal mesenchymal stem cells (MSCs). This study follows the rules of PRISMA and is registered in the Institutional Review Board of PROSPERO International of systematic reviews, numbered protocol code: CRD42020202465.<br />Method: The data search systematization was based on the words "mesenchymal stem cell" AND "immortalization." The search period for publications was between 2000 and 2022, and the databases used were SCOPUS, PUBMED, and SCIENCE DIRECT. The search strategies identified 384 articles: 229 in the SCOPUS database, 84 in PUBMED, and 71 in SCIENCE DIRECT. After screening by titles and abstracts, 285 articles remained. This review included thirty-nine articles according to the inclusion and exclusion criteria.<br />Result: In 28 articles, MSCs were immortalized from humans and 11 animals. The most used immortalization methodology was viral transfection. The most common immortalized cell type was the MSC from bone marrow, and the most used gene for immortalizing human and animal MSCs was hTERT (39.3%) and SV40T (54.5%), respectively.<br />Conclusion: Also, it was observed that although less than half of the studies performed tumorigenicity assays to validate the immortalized MSCs, other assays, such as qRT-PCR, colony formation in soft agar, karyotype, FISH, and cell proliferation, were performed in most studies on distinct MSC cell passages.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Details

Language :
English
ISSN :
2212-3946
Volume :
19
Issue :
7
Database :
MEDLINE
Journal :
Current stem cell research & therapy
Publication Type :
Academic Journal
Accession number :
38221663
Full Text :
https://doi.org/10.2174/011574888X268464231016070900