Back to Search
Start Over
Changes in the expression of mexB, mexY, and oprD in clinical Pseudomonas aeruginosa isolates.
- Source :
-
Proceedings of the Japan Academy. Series B, Physical and biological sciences [Proc Jpn Acad Ser B Phys Biol Sci] 2024; Vol. 100 (1), pp. 57-67. - Publication Year :
- 2024
-
Abstract
- Changes in expression levels of drug efflux pump genes, mexB and mexY, and porin gene oprD in Pseudomonas aeruginosa were investigated in this study. Fifty-five multidrug-resistant P. aeruginosa (MDRP) strains were compared with 26 drug-sensitive strains and 21 strains resistant to a single antibiotic. The effect of the efflux inhibitor Phe-Arg-β-naphthylamide on drug susceptibility was determined, and gene expression was quantified using real-time quantitative real-time reverse transcription polymerase chain reaction. In addition, the levels of metallo-β-lactamase (MBL) and 6'-N-aminoglycoside acetyltransferase [AAC(6')-Iae] were investigated. Efflux pump inhibitor treatment increased the sensitivity to ciprofloxacin, aztreonam, and imipenem in 71%, 73%, and 29% of MDRPs, respectively. MBL and AAC(6')-Iae were detected in 38 (69%) and 34 (62%) MDRP strains, respectively. Meanwhile, 76% of MDRP strains exhibited more than 8-fold higher mexY expression than the reference strain PAO1. Furthermore, 69% of MDRP strains expressed oprD at levels less than 0.01-fold of those in PAO1. These findings indicated that efflux pump inhibitors in combination with ciprofloxacin or aztreonam might aid in treating MDRP infections.
Details
- Language :
- English
- ISSN :
- 1349-2896
- Volume :
- 100
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Proceedings of the Japan Academy. Series B, Physical and biological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 38199247
- Full Text :
- https://doi.org/10.2183/pjab.100.006