N-(Heteroaryl)thiophene sulfonamides as angiotensin AT2 receptor ligands.

Two series of N-(heteroaryl)thiophene sulfonamides, encompassing either a methylene imidazole group or a tert-butylimidazolylacetyl group in the meta position of the benzene ring, have been synthesized. An AT ₂ R selective ligand with a K _i of 42 nM was identified in the first series and in the second series, six AT ₂ R selective ligands with significantly improved binding affinities and K _i values of <5 nM were discovered. The binding modes to AT ₂ R were explored by docking calculations combined with molecular dynamics simulations. Although some of the high affinity ligands exhibited fair stability in human liver microsomes, comparable to that observed with C21 undergoing clinical trials, most ligands displayed a very low metabolic stability with t _½ of less than 10 min in human liver microsomes. The most promising ligand, with an AT ₂ R K _i value of 4.9 nM and with intermediate stability in human hepatocytes (t _½  = 77 min) caused a concentration-dependent vasorelaxation of pre-contracted mouse aorta.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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