Back to Search
Start Over
Ultrafast Formation of a Delocalized Triplet-Excited State in an Epigenetically Modified DNA Duplex under Direct UV Excitation.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2024 Jan 24; Vol. 146 (3), pp. 1839-1848. Date of Electronic Publication: 2024 Jan 09. - Publication Year :
- 2024
-
Abstract
- Epigenetic modifications impart important functionality to nucleic acids during gene expression but may increase the risk of photoinduced gene mutations. Thus, it is crucial to understand how these modifications affect the photostability of duplex DNA. In this work, the ultrafast formation (<20 ps) of a delocalized triplet charge transfer (CT) state spreading over two stacked neighboring nucleobases after direct UV excitation is demonstrated in a DNA duplex, d(G <superscript>5f</superscript> C) <subscript>9</subscript> •d(G <superscript>5f</superscript> C) <subscript>9</subscript> , made of alternating guanine (G) and 5-formylcytosine ( <superscript>5f</superscript> C) nucleobases. The triplet yield is estimated to be 8 ± 3%, and the lifetime of the triplet CT state is 256 ± 22 ns, indicating that epigenetic modifications dramatically alter the excited state dynamics of duplex DNA and may enhance triplet state-induced photochemistry.
- Subjects :
- Ultraviolet Rays
DNA chemistry
DNA radiation effects
Epigenesis, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5126
- Volume :
- 146
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 38194423
- Full Text :
- https://doi.org/10.1021/jacs.3c04567