Back to Search
Start Over
Aggregated eosinophils and neutrophils characterize the properties of mucus in chronic rhinosinusitis.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2024 May; Vol. 153 (5), pp. 1306-1318. Date of Electronic Publication: 2024 Jan 04. - Publication Year :
- 2024
-
Abstract
- Background: Airway obstruction caused by viscous mucus is an important pathophysiologic characteristic of persistent inflammation, which can result in organ damage.<br />Objective: We investigated the hypothesis that the biophysical characteristics of accumulating granulocytes affect the clinical properties of mucus.<br />Methods: Surgically acquired nasal mucus samples from patients with eosinophilic chronic rhinosinusitis and neutrophil-dominant, noneosinophilic chronic rhinosinusitis were evaluated in terms of computed tomography density, viscosity, water content, wettability, and protein composition. Isolated human eosinophils and neutrophils were stimulated to induce the formation of extracellular traps, followed by the formation of aggregates. The biophysical properties of the aggregated cells were also examined.<br />Results: Mucus from patients with eosinophilic chronic rhinosinusitis had significantly higher computed tomography density, viscosity, dry weight, and hydrophobicity compared to mucus from patients with noneosinophilic chronic rhinosinusitis. The levels of eosinophil-specific proteins in mucus correlated with its physical properties. Eosinophil and neutrophil aggregates showed physical and pathologic characteristics resembling those of mucus. Cotreatment with deoxyribonuclease and heparin, which slenderizes the structure of eosinophil extracellular traps, efficiently induced reductions in the viscosity and hydrophobicity of both eosinophil aggregates and eosinophilic mucus.<br />Conclusions: The present study elucidated the pathogenesis of mucus stasis in infiltrated granulocyte aggregates from a novel perspective. These findings may contribute to the development of treatment strategies for eosinophilic airway diseases.<br /> (Copyright © 2024 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Aged
Female
Humans
Male
Middle Aged
Cell Aggregation
Chronic Disease
Nasal Mucosa immunology
Nasal Mucosa pathology
Viscosity
Eosinophils immunology
Extracellular Traps immunology
Extracellular Traps metabolism
Mucus metabolism
Neutrophils immunology
Rhinosinusitis immunology
Rhinosinusitis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-6825
- Volume :
- 153
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38181841
- Full Text :
- https://doi.org/10.1016/j.jaci.2023.11.925