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Optimization of media components for enhanced carotenoid production by Paracoccus marcusii RSPO1 and assessment of their cytotoxicity against A549 and vero cells.
- Source :
-
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Jul; Vol. 54 (6), pp. 764-778. Date of Electronic Publication: 2024 Jan 02. - Publication Year :
- 2024
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Abstract
- In this study, we tried to explore the influence of various tricarboxylic acid (TCA) cycle intermediates on carotenoid production and with a focus on enhancing pigment biosynthesis, we conducted two statistical analysis. In case of TCA intermediates influence on pigment production by Paracoccus marcusii RSPO1; fumaric acid, and malic acid were observed as potent enhancers of pigment biosynthesis. Further, to optimize key media components for enhanced carotenoid production, the Plackett-Burman design was employed encompassing carbon, nitrogen sources, TCA cycle intermediates, and metal salts. The selected factors after Plackett Burman were fine-tuned through Response Surface Methodology and the optimal concentrations that have remarkably elevated carotenoid production were starch-2.24 g/l, MgSO <subscript>4</subscript> -0.416 g/l, ZnSO <subscript>4</subscript> -0.0157 g/l, and fumaric Acid-16 mM. Further, evaluation of pigment cytotoxicity against normal (Vero) and Non-Small Cell Carcinoma (A549) cells was performed. The resultant IC <subscript>50</subscript> values were quantified as 161.3 µg/ml and 7.623 µg/ml for Vero and A549 cells, respectively. Moreover, a reactive oxygen species (ROS) determination study in A549 cells was done which have shown a noteworthy threefold ROS production in A549 cells through fluorescence spectroscopic observation. This implies that the bacterial carotenoids can act as potent pro-oxidants against cancerous cells while being nontoxic toward normal cells.
Details
- Language :
- English
- ISSN :
- 1532-2297
- Volume :
- 54
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Preparative biochemistry & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 38165781
- Full Text :
- https://doi.org/10.1080/10826068.2023.2282533