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Development of Organoids to Study Infectious Host Interactions.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2742, pp. 151-164. - Publication Year :
- 2024
-
Abstract
- Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2742
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 38165622
- Full Text :
- https://doi.org/10.1007/978-1-0716-3561-2_12