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Development of Organoids to Study Infectious Host Interactions.

Authors :
Lahree A
Gilbert L
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2742, pp. 151-164.
Publication Year :
2024

Abstract

Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2742
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
38165622
Full Text :
https://doi.org/10.1007/978-1-0716-3561-2_12