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Molecular B-cell clonality assay in minor salivary glands as a useful tool for the lymphoma risk assessment in Sjögren's syndrome.
- Source :
-
Joint bone spine [Joint Bone Spine] 2024 May; Vol. 91 (3), pp. 105686. Date of Electronic Publication: 2023 Dec 29. - Publication Year :
- 2024
-
Abstract
- Objectives: Non-Hodgkin's lymphoma (NHL) risk assessment is crucial in Sjögren's syndrome (SS). We studied the prevalence of clonal immunoglobulin gene rearrangements in minor salivary glands (MSG) and their correlations with lymphoma occurrence and with previously established NHL predictors.<br />Methods: Molecular B-cell expansion was studied in fresh-frozen MSG of 207 patients with either suspected SS or with suspected lymphoma during SS, using a standardised multiplex PCR assay combined with heteroduplex analysis by microcapillary electrophoresis. The assignation of clonal cases was based on EuroClonality consortium guidelines.<br />Results: Among 207 studied patients, 31 (15%) had MSG monoclonal B-cell infiltration. Monoclonality was significantly more frequent in patients with SS (28/123, 22.8%) compared with patients without SS (3/84, 3.6%, P<0.001). Monoclonal B-cell infiltration in MSG of SS patients correlated significantly with ongoing salivary gland NHL, salivary gland swelling, CD4 <superscript>+</superscript> T-cell lymphopenia, rheumatoid factor (RF) activity, low complement levels and type 2 mixed cryoglobulinemia. The accumulation of biological risk factors was associated with a higher rate of MSG B-cell monoclonality given that patients with only positive RF had no probability of MSG B-cell monoclonality, RF-positive patients with 1 or 2 other risk factors had a 25.0% and 85.7% probability of MSG B-cell monoclonality, respectively.<br />Conclusion: The detection of MSG monoclonal B-cell expansion by this easy-to-perform molecular assay is useful, both at the time of diagnosis and during the course of SS. Monoclonal B-cell expansion is associated with a subset of SS patients presenting either ongoing lymphoma or other established lymphoma predictive factors.<br /> (Copyright © 2023 Société française de rhumatologie. Published by Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Humans
Female
Middle Aged
Risk Assessment methods
Male
Aged
Adult
Lymphoma, Non-Hodgkin pathology
Lymphoma, Non-Hodgkin diagnosis
Lymphoma, Non-Hodgkin genetics
Lymphoma, Non-Hodgkin immunology
Salivary Gland Neoplasms genetics
Salivary Gland Neoplasms pathology
Salivary Gland Neoplasms immunology
Aged, 80 and over
Sjogren's Syndrome immunology
Sjogren's Syndrome diagnosis
Sjogren's Syndrome genetics
B-Lymphocytes immunology
Salivary Glands, Minor pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1778-7254
- Volume :
- 91
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Joint bone spine
- Publication Type :
- Academic Journal
- Accession number :
- 38161050
- Full Text :
- https://doi.org/10.1016/j.jbspin.2023.105686