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Effect of Silicone Patch Containing Metal-organic Framework on Hypertrophic Scar Suppression.
- Source :
-
In vivo (Athens, Greece) [In Vivo] 2024 Jan-Feb; Vol. 38 (1), pp. 235-245. - Publication Year :
- 2024
-
Abstract
- Background/aim: Hypertrophic scars (HS) are an abnormal cutaneous condition of wound healing characterized by excessive fibrosis and disrupted collagen deposition. This study assessed the potential of a silicone patch embedded with chemically stable zirconium-based metal-organic frameworks (MOF)-808 structures to mitigate HS formation using a rabbit ear model.<br />Materials and Methods: A silicone patch was strategically engineered by incorporating Zr-MOF-808, a composite structure comprising metal ions and organic ligands. Structural integrity of the Zr-MOF-808 silicone patch was validated using scanning electron microscopy and X-ray diffraction analysis. The animals were divided into three groups: a control, no treatment group (Group 1), a silicone patch treatment group (Group 2), and a group treated with a 0.2% loaded Zr-MOF-808 silicone patch (Group 3). HS suppression effects were quantified using scar elevation index (SEI), dorsal skin thickness measurements, and myofibroblast protein expression.<br />Results: Histopathological examination of post-treatment HS samples revealed substantial reductions in SEI (34.6%) and epidermal thickness (49.5%) in Group 3. Scar hyperplasia was significantly diminished by 53.5% (p<0.05), while collagen density declined by 15.7% in Group 3 compared to Group 1. Western blot analysis of protein markers, including TGF-β1, collagen-1, and α-SMA, exhibited diminished levels by 8.8%, 12%, and 21.3%, respectively, in Group 3, and substantially higher levels by 21.9%, 27%, and 39.9%, respectively, in Group 2. On the 35th day post-wound generation, Zr-MOF-808-treated models exhibited smoother, less conspicuous, and flatter scars.<br />Conclusion: Zr-MOF-808-loaded silicone patch reduced HS formation in rabbit ear models by inducing the proliferation and remodeling of the wound healing process.<br /> (Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Animals
Rabbits
Fibroblasts
Collagen Type I metabolism
Collagen Type I pharmacology
Collagen metabolism
Transforming Growth Factor beta1 metabolism
Transforming Growth Factor beta1 pharmacology
Cicatrix, Hypertrophic metabolism
Cicatrix, Hypertrophic pathology
Metal-Organic Frameworks metabolism
Metal-Organic Frameworks pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7549
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- In vivo (Athens, Greece)
- Publication Type :
- Academic Journal
- Accession number :
- 38148076
- Full Text :
- https://doi.org/10.21873/invivo.13430