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Examining Phosphatases Through Immunofluorescent Microscopy.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2743, pp. 111-122. - Publication Year :
- 2024
-
Abstract
- Immunofluorescent microscopy enables the examination of cellular expression and localization of proteins. Cellular localization can often impact protein function, as certain molecular interactions occur in specific cellular compartments. Here we describe in detail the processes necessary for identifying phosphatases in the cell through immunofluorescent microscopy. Identification of phosphatase expression and localization could lead to the discovery of protein function in disease states along with potential substrates and binding partners.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Microscopy, Fluorescence
Phosphoric Monoester Hydrolases
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2743
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 38147211
- Full Text :
- https://doi.org/10.1007/978-1-0716-3569-8_7