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A biocompatible chitosan-based fluorescent polymer for efficient H 2 O 2 detection in living cells and water samples.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2024 Feb; Vol. 257 (Pt 2), pp. 128760. Date of Electronic Publication: 2023 Dec 14. - Publication Year :
- 2024
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Abstract
- As a biomarker of oxidative stress, hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) plays a complex role in organisms, including regulating cell signaling, respiration, the immune system, and other life processes. Therefore, it is important to develop a tool that can simply and effectively monitor H <subscript>2</subscript> O <subscript>2</subscript> levels in organisms and the environment. In this work, naphthalene fluorophores with a borate structure were introduced into chitosan (CTS) azide, and a CTS-based fluorescence sensor (CTS-HP) was designed for sensitive H <subscript>2</subscript> O <subscript>2</subscript> detection. The biocompatibility and degradability of CTS endowed CTS-HP with reduced biotoxicity compared with organic fluorescent dyes, and the substitution degree of fluorophores on the CTS chains was 0.703. The randomly coiled chain structure of the CTS-HP probe enabled the boronic acid recognition sites on the fluorophores to achieve the enrichment of analyte H <subscript>2</subscript> O <subscript>2</subscript> through a synergistic effect. Therefore, the probe CTS-HP (10 μg mL <superscript>-1</superscript> ) exhibited a 21-fold fluorescence enhancement and good detection limit (LOD = 8.98 nM) in H <subscript>2</subscript> O <subscript>2</subscript> solution, reaching the maximum fluorescence response faster (within 16 min). The probe also successfully achieved the fluorescence imaging of endogenous and exogenous H <subscript>2</subscript> O <subscript>2</subscript> in zebrafish and living cells and labeled the recovery experiment of H <subscript>2</subscript> O <subscript>2</subscript> in real water samples (recoveries rates of 90.93-102.9 % and RSD < 3.09 %).<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 257
- Issue :
- Pt 2
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 38103662
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2023.128760