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Protocol for sorting and culturing of mouse early erythroid progenitor BFU-E cells.
- Source :
-
STAR protocols [STAR Protoc] 2024 Mar 15; Vol. 5 (1), pp. 102718. Date of Electronic Publication: 2023 Dec 12. - Publication Year :
- 2024
-
Abstract
- Techniques allowing the long-term culture of the burst-forming unit of erythroid (BFU-E) progenitor cells are essential for understanding erythropoiesis. Here, we present a protocol for sorting mouse BFU-E cells and culturing them in a medium that promotes BFU-E cell expansion. We describe steps for isolating BFU-E cells from mouse fetal livers by combining magnetic microbeads with flow cytometry and culturing BFU-E cells with a specific expansion media. This approach can enhance the production of BFU-E cells. For complete details on the use and execution of this protocol, please refer to Li et al.. <superscript>1</superscript> .<br />Competing Interests: Declaration of interests We have a Chinese patent pending review (2023105116380).<br /> (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 38088932
- Full Text :
- https://doi.org/10.1016/j.xpro.2023.102718