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Structural organization of the retriever-CCC endosomal recycling complex.
- Source :
-
Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Jun; Vol. 31 (6), pp. 910-924. Date of Electronic Publication: 2023 Dec 07. - Publication Year :
- 2024
-
Abstract
- The recycling of membrane proteins from endosomes to the cell surface is vital for cell signaling and survival. Retriever, a trimeric complex of vacuolar protein-sorting-associated protein (VPS)35L, VPS26C and VPS29, together with the CCC complex comprising coiled-coil domain-containing (CCDC)22, CCDC93 and copper metabolism domain-containing (COMMD) proteins, plays a crucial role in this process. The precise mechanisms underlying retriever assembly and its interaction with CCC have remained elusive. Here, we present a high-resolution structure of retriever in humans determined using cryogenic electron microscopy. The structure reveals a unique assembly mechanism, distinguishing it from its remotely related paralog retromer. By combining AlphaFold predictions and biochemical, cellular and proteomic analyses, we further elucidate the structural organization of the entire retriever-CCC complex across evolution and uncover how cancer-associated mutations in humans disrupt complex formation and impair membrane protein homeostasis. These findings provide a fundamental framework for understanding the biological and pathological implications associated with retriever-CCC-mediated endosomal recycling.<br /> (© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)
- Subjects :
- Humans
Models, Molecular
Vesicular Transport Proteins metabolism
Vesicular Transport Proteins chemistry
Vesicular Transport Proteins ultrastructure
Vesicular Transport Proteins genetics
Cation Transport Proteins chemistry
Cation Transport Proteins metabolism
Cation Transport Proteins ultrastructure
Protein Conformation
Multiprotein Complexes chemistry
Multiprotein Complexes metabolism
Multiprotein Complexes ultrastructure
Endosomes metabolism
Endosomes ultrastructure
Cryoelectron Microscopy
Subjects
Details
- Language :
- English
- ISSN :
- 1545-9985
- Volume :
- 31
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Nature structural & molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 38062209
- Full Text :
- https://doi.org/10.1038/s41594-023-01184-4