Melatonin-regulated heat shock proteins and mitochondrial ATP synthase induce drought tolerance through sustaining ROS homeostasis in H 2 S-dependent manner.

Drought is thought to be one of the major global hazards to crop production. Understanding the role of melatonin (Mel) during plant adaptive responses to drought stress (DS) was the aim of the current investigation. Involvement of hydrogen sulfide (H ₂ S) was also explored in Mel-regulated mechanisms of plants' tolerance to DS. A perusal of the data shows that exposure of tomato plants to DS elevated the activity of mitochondrial enzymes viz. pyruvate dehydrogenase, malate dehydrogenase, and citrate synthase. Whereas the activity of ATP synthase and ATPase was downregulated under stress conditions. Under DS, an increase in the expression level of heat shock proteins (HSPs) and activation level of antioxidant defense system was observed as well. On the other hand, an increase in the activity of NADPH oxidase and glycolate oxidase was observed along with the commencement of oxidative stress and accompanying damage. Application of 30 μM Mel to drought-stressed plants enhanced H ₂ S accumulation and further elevated the activity of mitochondrial enzymes, activation level of the defense system, and expression of HSP17.6 and HSP70. Positive effect of Mel on these attributes was reflected by reduced level of ROS and related damage. However, application of H ₂ S biosynthesis inhibitor DL-propargylglycine reversed the effect of Mel on the said attributes and again the damaging effects of drought were observed even in presence of Mel. This observation led us to conclude that Mel-regulated defense mechanisms operate through endogenous H ₂ S under DS conditions.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Masson SAS. All rights reserved.)