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Lacticaseibacillus casei decrease long-chain fatty acids and most substances in an experimental model of intestinal mucositis.

Authors :
Barbosa SJA
Silva ASD
Oliveira MMB
Ribeiro SB
Medeiros CACX
Ferreira LS
Pinheiro FASD
Sousa Junior FC
Martins AA
Araújo Júnior RF
Garcia VB
Araújo AA
Source :
Acta cirurgica brasileira [Acta Cir Bras] 2023 Dec 01; Vol. 38, pp. e386723. Date of Electronic Publication: 2023 Dec 01 (Print Publication: 2023).
Publication Year :
2023

Abstract

Purpose: To evaluate the long-chain fatty acid and major compounds levels in the feces after prophylactic oral use of Lacticaseibacillus casei in an experimental model of intestinal mucositis.<br />Methods: Fifteen Swiss mice were randomly divided into three groups (n=5/group): The negative or positive control groups (n = 5) received saline orally for 18 days and an the intraperitoneal (i.p.) of saline or 5 Fluorouracil (450 mg/kg) in 15th day, respectability. L. casei group received oral concentration of L. casei (1x109 CFU/mL) for 18 days, the i.p. injection of 5-fluorouracil (450 mg/kg) in 15th days. Tissue samples from colon and each small intestine segment were collected for histopathological analysis. Stool samples were collected. Fecal composition of long-chain fatty acids and sterols were analysed by gas chromatography-mass spectrometry on the 15th and the 18th day.<br />Results: The mucosa layer of all small intestine segments of animals from L. casei showed well preserved epithelium and glands, without necrosis signs, but Goblet cells number decreased. Several long-chain fatty acids and sterols have been identified before and after in the groups. L. casei administration after 5-FU treatment reduced concentrations of linoleic acid (18:2) (p < 0.001) and oleic acid (18:1) (p < 0.001) in feces.<br />Conclusions: L. casei prevented the mucosal damage associated with 5-FU-induced intestinal mucositis reduced long-chain fatty acid levels in the feces.

Details

Language :
English
ISSN :
1678-2674
Volume :
38
Database :
MEDLINE
Journal :
Acta cirurgica brasileira
Publication Type :
Academic Journal
Accession number :
38055402
Full Text :
https://doi.org/10.1590/acb386723