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Anthocyanins Inhibits Oxidative Injury in Human Retinal Pigment Epithelial ARPE-19 Cells via Activating Heme Oxygenase-1.

Authors :
Park C
Hwangbo H
Kim SO
Noh JS
Park SH
Hong SH
Hong SH
Kim GY
Choi YH
Source :
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2024 Mar 28; Vol. 34 (3), pp. 596-605. Date of Electronic Publication: 2023 Nov 13.
Publication Year :
2024

Abstract

Anthocyanins belong to phenolic pigments and are known to have various pharmacological activities. This study aimed to investigate whether anthocyanins could inhibit hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> )-induced oxidative damage in human retinal pigment epithelial ARPE-19 cells. Our results indicated that anthocyanins suppressed H <subscript>2</subscript> O <subscript>2</subscript> -induced genotoxicity, while inhibiting reactive oxygen species (ROS) production and preserving diminished glutathione. Anthocyanins also suppressed H <subscript>2</subscript> O <subscript>2</subscript> -induced apoptosis by reversing the Bcl-2/Bax ratio and inhibiting caspase-3 activation. Additionally, anthocyanins attenuated the release of cytochrome c into the cytosol, which was achieved by interfering with mitochondrial membrane disruption. Moreover, anthocyanins increased the expression of heme oxygenase-1 (HO-1) as well as its activity, which was correlated with the phosphorylation and nuclear translocation of nuclear factor-erythroid-2 related factor 2 (Nrf2). However, the cytoprotective and anti-apoptotic effects of anthocyanins were significantly attenuated by the HO-1 inhibitor, demonstrating that anthocyanins promoted Nrf2-induced HO-1 activity to prevent ARPE-19 cells from oxidative stress. Therefore, our findings suggest that anthocyanins, as Nrf2 activators, have potent ROS scavenging activity and may have the potential to protect ocular injury caused by oxidative stress.

Details

Language :
English
ISSN :
1738-8872
Volume :
34
Issue :
3
Database :
MEDLINE
Journal :
Journal of microbiology and biotechnology
Publication Type :
Academic Journal
Accession number :
38044685
Full Text :
https://doi.org/10.4014/jmb.2310.10011